| abstract |
The present invention provides a protein applied to NK cell culture, a medium formulation combination and a preparation method, and an application of the protein. The protein and medium formula combination of the present invention can avoid the contamination of trophoblast cells by exogenous cell lines, and at the same time improve the efficiency and stability of NK culture, and cultivate NK cells with high purity and high adaptability. One aspect of the present invention provides a protein applied to NK cell culture, the protein is MICA-Ig, and the MICA-Ig is a fusion of human MICA protein on the surface of tumor cells and the Fc segment of human immunoglobulin IgG1 into recombinant MICA-Ig protein. |