http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109627326-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 |
filingDate | 2019-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109627326-B |
titleOfInvention | Extraction method of non-denatured collagen and identification method of collagen |
abstract | The invention belongs to the technical field of collagen, and particularly relates to a method for extracting non-denatured collagen and a method for identifying collagen. The invention provides a method for extracting non-denatured collagen, which comprises the following steps: a) adding collagen into the incubation liquid for incubation and centrifuging to obtain insoluble substances; b) sequentially carrying out enzymolysis, neutralization and salting-out on the insoluble substances to obtain non-denatured collagen; wherein the incubation solution is selected from a Tris-HCl solution, a Bis-Tris solution, an imidazole buffer solution or a trypsin culture solution. The experimental result shows that the incubation liquid is adopted to incubate the collagen, so that the denatured collagen can be removed, the non-denatured collagen is extracted, and the activity of the collagen product can be improved. |
priorityDate | 2019-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.