http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109613092-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54373 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5438 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-48 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 |
| filingDate | 2018-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109613092-B |
| titleOfInvention | A biosensor that simultaneously detects the total amount of specific DNA and the amount of single-base mutation and its detection method |
| abstract | The invention discloses a biosensor for simultaneously detecting the total amount of specific DNA and the mutation amount of a single base and a detection method thereof. The sulfhydryl-modified capture strand cDNA designed with 6 pairs of complementary bases is pre-hybridized with the methylene blue-modified beacon-capture strand pcDNA to obtain a double helix at the bottom and two unpaired long chains as the capture arms at the top. "Anchored" alDNA is assembled on the surface of the gold electrode and the vacant sites where alDNA is not assembled on the electrode surface are blocked to obtain a biosensor interface; the sensor first captures the total amount of DNA of a specific sequence, and then undergoes specific enzymatic-chemical denaturation , the content of specific single-base mutant DNA can be obtained. The invention has simple construction method and high detection sensitivity, can simultaneously quantitatively detect the total amount of specific DNA and the content of mutant DNA, and has mild reaction conditions, stable and efficient sensing system, and is expensive and cumbersome compared with clinical gene sequencing. The practical significance and development potential are conducive to the promotion and use. |
| priorityDate | 2018-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.