http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109610008-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e770006a93254a4c7894446b7175f23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7458b66d235f9eb46384c22911164030 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate | 2018-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff71defd290e2118c9a0c8e2922a8e75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a9b3c3d523c0e89b598f711f92aea6f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf4eb65daa9a2d4b464227e6ac8a28e9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c91bdf390758fba9d4d9571f322a91c3 |
| publicationDate | 2019-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109610008-A |
| titleOfInvention | Library construction method, detection method and kit for central system infection pathogen detection based on high-throughput sequencing |
| abstract | A high-throughput sequencing-based library construction method, detection method and kit for central system infection pathogen detection, the library construction method comprises: extracting DNA from a sample and fragmenting the DNA to obtain a fragmented DNA sequence; The sequence is added to the end repair system to carry out the end repair reaction. After the end repair reaction is completed, the linker sequence, ligase, ATP and polyethylene glycol are directly added to the reaction system. Under the action of the ligase, ATP is used as a coenzyme to catalyze the linker sequence connection. The two ends of the end repair reaction product are obtained to obtain a linker ligation product; after the linker ligation product is purified, nucleic acid single strands complementary to both ends of the linker sequence are added as primers for PCR amplification. The method can realize rapid and broad-spectrum detection of pathogenic microorganisms in central system infections, without the need to predict too much clinical information of the test samples, it can be detected quickly and efficiently, the test results are unbiased, and it can also solve the failure rate of low-concentration cerebrospinal fluid sample library construction high question. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111455024-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114015757-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111394486-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113201599-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110349630-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020248110-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111020018-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110349630-B |
| priorityDate | 2018-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 78.