http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109609507-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1048 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-115 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-115 |
| filingDate | 2016-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109609507-B |
| titleOfInvention | Single-stranded DNA aptamer of phthalate plasticizer, screening and characterization method of single-stranded DNA aptamer and electrochemical sensor |
| abstract | The invention relates to a phthalic acid ester plasticizer DNA aptamer, a screening and characterization method thereof and an electrochemical sensor. The method of the invention comprises the following steps: the method comprises the following steps: preparing a dibutyl phthalate derivative having an amino group at the terminal of one of linear fatty side chains by chemical synthesis; step two: covalently attaching DBP-NH2 to the agarose microspheres by condensation reaction of amino groups with oxirane activating groups on the agarose microspheres; step three: performing solid phase separation-based screening of nucleic acid aptamers; step four: after screening, carrying out high-throughput sequencing on the obtained enrichment library; step five: performing affinity and selectivity tests on two sequences DBP-1 and DBP-2 with the highest occurrence frequency in high-throughput sequencing; step six: and constructing the electrochemical sensor based on the DBP-1. The invention can be used for developing various detection technologies and biosensors, and has important application value for convenient detection of phthalate plasticizers in foods or environments. |
| priorityDate | 2016-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 81.