| abstract |
The present invention discloses a set of HDACs aptamer, belongs to genetic engineering, immunology and oncology technical field.The aptamers are one or several any sequence in nucleotide sequence shown in NO:1 ~ 5 SEQ ID.Aptamer of the present invention is obtained using Protein S ELEX technology screening, and molecular weight is smaller, is readily synthesized and modifies, can high specific identification and high-affinity combination HDACs, in conjunction with other albumen not occurrence features, non-immunogenicity, stablize easily modification, convenient for synthesis and saves.The HDACs detection kit and reagent paper prepared with the aptamers is high to HDACs detection sensitivity, and is easy to optimize.It is applied in diagnosing tumor, tumor prognosis and the assessment of monitoring, diagnosing tumor. |