| abstract |
The invention relates to a tree shrew microglia cell culture medium and a method for separating, culturing and purifying it in vitro, and belongs to the field of biotechnology. The medium of the present invention takes DMEM/F12 complete medium as the basal medium, and adds 10% fetal bovine serum, 1% double antibody, 1% MGS and 0.04% B27 to the basal medium. The present invention also provides a method for in vitro separation, culture and purification of tree shrew microglia cell bodies. In the steps of separating and purifying glial cells, applying the method for separating, culturing and purifying tree shrew microglia in vitro of the present invention can cultivate tree shrew microglia with high purity and strong vitality, which can basically meet the requirements of medicine, biology, etc. Requirements for microglia-related experimental research in the research field. |