http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109608516-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-145 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2019-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109608516-B |
| titleOfInvention | Rapid purification method of royal jelly major proteins 1, 2 and 3 |
| abstract | The rapid purification method of the royal jelly major proteins 1, 2 and 3 comprises the following steps: dissolving Lac Regis Apis in buffer solution A with pH of 7, and shaking overnight; centrifuging the mixed solution at low temperature, and filtering the supernatant with a filter membrane; loading the supernatant onto a pre-packed weak cation chromatographic column equilibrated with buffer A; washing the column with buffer solution A at a predetermined flow rate to obtain royal jelly major protein 1; mixing the buffer solution A and the buffer solution B in proportion by a gradient mixer of the chromatograph, gradually increasing the proportion of the buffer solution B, performing gradient elution on the column, and sequentially eluting the main proteins 2 and 3 of the royal jelly combined on the column. The rapid purification method of the main proteins 1, 2 and 3 of the royal jelly has the advantages of good repeatability, high yield, simple operation, convenience and rapidness, cascade amplification, full satisfaction of scientific research and scale production, overcoming the defects of time and labor consumption, high cost, low yield and the like of the traditional separation method, and optimizing the extraction process of the main proteins of the royal jelly. |
| priorityDate | 2019-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.