http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109593750-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-01084 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2019-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109593750-B |
| titleOfInvention | Nitrile hydratase mutant, genetic engineering bacterium containing same and application thereof |
| abstract | The invention discloses a nitrile hydratase mutant, a genetic engineering bacterium containing the mutant and application of the nitrile hydratase mutant, and belongs to the technical field of enzyme engineering. According to the invention, the 47 th glycine of a nitrile hydratase mutant alpha L6T/A19V/F126Y-beta M46K/E108R/S212Y (disclosed in the invention patent CN102216455A) is mutated into asparagine, and the obtained new mutant enzyme has better temperature tolerance and product tolerance, and is beneficial to subsequent industrial production. The recombinant strain containing the nitrile hydratase mutant is subjected to high-density fermentation, and the nicotinamide is prepared by whole-cell catalytic reaction with nicotinonitrile as a substrate. Compared with a chemical production method, the method has the advantages of safe and clean production process and no environmental pollution, and compared with an enzyme method, the method has the advantages of cheap substrate, high catalytic efficiency, over 95 percent of yield of the final product nicotinamide and concentration of 680g/L, and simplifies the steps of separation and purification of the product. |
| priorityDate | 2019-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 370.