http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109593702-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0018 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-0102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0004 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2019-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109593702-B |
| titleOfInvention | Method for synthesizing L-phenyllactic acid by whole cell transformation of genetic engineering strain |
| abstract | The invention discloses a method for synthesizing L-phenyllactic acid by whole cell transformation of a genetic engineering strain, and particularly relates to a method capable of realizing cofactor NAD + A gene engineering strain for coexpressing phenylalanine dehydrogenase and L-hydroxyisocaproate reductase with NADH self-circulation, a method for synthesizing L-phenyllactic acid by converting a substrate L-phenylalanine through whole cells, belonging to the technical field of biological engineering. The L-phenyllactic acid is synthesized by the whole cell transformation of a recombinant strain E.coli BL21(DE3)/pET28a-pdh-ldh, and the whole cell transformation rate can reach 88.9-95.6% under the action of adding a surfactant. The method realizes cofactor NAD + And NADH self-circulation, reduces the addition of the cofactor, reduces the production cost, and has wide application prospect in the field of industrial synthesis of L-phenyllactic acid. |
| priorityDate | 2019-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 356.