http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109593694-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2018-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109593694-B |
| titleOfInvention | Ngpiwi protein-mediated bovine-derived escherichia coli gene knockout strain and construction method thereof |
| abstract | The invention discloses a bovine-derived escherichia coli gene knockout strain based on Ngpii protein mediation and a construction method thereof; the bovine-derived escherichia coli gene knockout strain is a deletion strain which deletes ORF sequences or partial sequences of potential virulence related genes on bovine-derived escherichia coli genomes. The method comprises the steps of firstly, successfully constructing a recombinant plasmid with Ngpiwi and left and right homologous arms of a target gene sequence to be deleted; secondly, the recombinant plasmid is chemically transformed into bovine Escherichia coli, passage induction double exchange recombination is carried out at 28 ℃, a strain with a deleted target gene sequence is screened out by PCR, and then plasmid loss is induced at 37 ℃ in an antibiotic-free culture medium, so that the strain with a deleted potential virulence gene related sequence is obtained. The genetic operation system has the advantages of small plasmid, easy operation, wide application, no potential off-target effect, high knockout efficiency and no resistance gene screening marker, and provides an excellent tool for the research and development of bovine-derived escherichia coli genetic engineering vaccines. |
| priorityDate | 2018-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.