http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109576363-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-106 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2019-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109576363-B |
| titleOfInvention | Primer design method and kit for detecting polymorphism of human ABCC2 gene |
| abstract | The invention discloses a primer design method and a kit for detecting polymorphism of human ABCC2 gene, belonging to the technical field of clinical laboratory and molecular biology. A primer design method for gene polymorphism detection meets the following conditions: (1) making the gene polymorphism site located at the middle position, designing the upstream and downstream primers by using the gene sequence with the length of 400bp before and after the site; (2) in a PCR reaction system, the final concentration of the primer is 1-3 mu M; (3) the total length of each product is less than or equal to 160 bp; (4) the annealing temperature Tm is 58-62 ℃; (5) purifying the synthesized primer sequence by HPLC; (6) mg in the system 2+ The final concentration of (B) is 2.0 to 2.5 mM. The invention has the advantages that: the primer design method avoids the interference of detecting gene polymorphism by a high-resolution melting curve technology; the ABCC2 gene kit has the advantages of direct typing during amplification, high flux, high speed, high accuracy and high specificity, can be used for searching a new target point of individualized difference, and provides basis for clinical individualized medication decision. |
| priorityDate | 2019-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.