http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109554327-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 |
| filingDate | 2018-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109554327-B |
| titleOfInvention | Method for separating and purifying amomum villosum mesophyll protoplast |
| abstract | The invention discloses a method for separating and purifying amomum villosum mesophyll protoplasts, which comprises the steps of preparing and pretreating materials, separating the protoplasts by adopting an enzymolysis method, purifying the protoplasts by adopting a sedimentation method and a drifting method. According to the invention, through researching the factors influencing the yield and activity of the protoplast, such as whether the material is pretreated, the types and concentration of enzymes, the enzymolysis time, the concentration of an osmotic pressure regulator and the influence of different purification methods on the purification effect of the mesophyll cell protoplast, the experimental conditions for separating and purifying the actinolite mesophyll protoplast are optimized, so that the high-yield and high-activity actinolite protoplast is obtained, and a foundation is laid for cultivating new varieties of actinolite through somatic cell fusion. |
| priorityDate | 2018-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 83.