http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109536616-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-124 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 |
| filingDate | 2018-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109536616-B |
| titleOfInvention | SNP (Single nucleotide polymorphism) marker and copy number variation method for screening female parent of high-fertility mauremys mutica |
| abstract | The invention belongs to the technical field of molecular marker assisted breeding, and discloses a method for screening SNP markers and copy number variation of female parent of high-fertility mauremys mutica, which comprises the following steps: obtaining DNA of a candidate female parent; screening ND1 fragment primers of 3 SNP sites and ND4 and ATP6 copy number primers; obtaining an ND1 fragment by PCR amplification and introducing the ND4 and ATP6 amplification fragments into a plasmid; sequencing and obtaining the copy number of ND4 and ATP6 according to the concentration of the plasmid; and (4) screening high-fertility individuals according to the SNP sites and the copy numbers of ND4 and ATP 6. The invention utilizes the multiplex PCR technology to carry out paternity test and determine the reproductive capacity of each female parent; according to the arrangement of the reproductive capacity from high to low, female turtles with the highest reproductive capacity are selected to construct a high-reproductive capacity group; the invention can be used for constructing basic groups to carry out subsequent fine variety breeding work. |
| priorityDate | 2018-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.