http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109526750-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109526750-B |
titleOfInvention | Somatic embryogenesis and plant regeneration method for rubicuna yunnanensis |
abstract | The invention discloses a method for embryogenesis and plant regeneration of a glaucomatous jade dew body cell, which comprises the following steps: the invention obtains the crystal jade dew by disinfection and cleaning, blade embryonic callus induction, embryonic callus maturation and differentiation, differentiation bud rooting induction and transplantation domestication, the invention takes the upper part of leaves as an explant, and the proper culture medium for embryonic callus induction is MS +6-BA (1.0-2.0 mg.L. L.) ‑1 )+NAA(0.1mg·L ‑1 )+2,4‑D(0.2mg·L ‑1 ) + agar (6 g. L) ‑1 ) + sucrose (30 mg. L ‑1 ) (ii) a The somatic embryo maturation and differentiation culture medium is MS +6-BA (1.0 mg. L) ‑1 )+NAA(0.1mg·L ‑1 ) + agar (6 g. L) ‑1 ) + sucrose (30 g. L) ‑1 ) 1/2MS Medium with IBA1.0g.L ‑1 Agar 6 g. L ‑1 And sucrose 30 g.L ‑1 Rooting induction is carried out on the somatic embryo differentiated seedling, the induction rate is up to 76%, and the addition of activated carbon in the culture medium is not beneficial to rooting induction. |
priorityDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.