| abstract |
The invention discloses a type A and type B flu virus fast classification test kit and its application. Including the throat swab and blood sample RNA fast extract reagent, PCR amplification reagent, and control reagent; PCR amplification reagents include PCR reaction buffer 1. Enzyme system 3, INFA/Infb primers; RNA fast extraction reagents are made of 0.1 % to 1.0 % (W/V) NLS, 300mm to 800mm DTT, 1 × Te Buffer; PCR reaction buffer 1 includes 5*RT‑PCR reaction buffer (50mm tris‑hcl, 75mm KCL, 3mm MgCl2, 10mm DTT), DNTPS, MGCL2; enzyme 3 includes HS Hi TAQ DNA polymerase, Super‑m‑mlV reverse enzyme inhibitor; The needle hybrid fluid includes the probe of the type A virus detection primer and the type B flu virus detection primer. |