http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109517881-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2018-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109517881-B |
| titleOfInvention | High-throughput sequencing library construction method for body fluid trace free RNA |
| abstract | The invention discloses a method for constructing a high-throughput sequencing library of trace free RNA in body fluid. The method comprises the following steps: purifying body fluid sample exRNA by using a silicon matrix, dephosphorylating, adding polyA tail, and then carrying out reverse transcription on the tailing product by using template conversion and locked nucleic acid technology to obtain cDNA with a joint at the 5' end; then low-cycle PCR is carried out, and finally a high-throughput sequencing library is obtained. By applying template conversion and nucleic acid locking technologies, the invention deeply optimizes the problems of trace RNA in the construction process of a high-throughput sequencing library, and greatly reduces the library construction time and cost. By applying the technology of the invention, a library can be built for trace RNA less than 10ng in one day, then data with good quality can be obtained through high-throughput sequencing, and a plurality of existing RNA biomarkers related to diseases can be found through analysis. |
| priorityDate | 2018-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.