http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109517835-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12G3-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-905 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12G3-022 |
| filingDate | 2018-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109517835-B |
| titleOfInvention | Method for reducing yellow wine yeast urea accumulation by localizing Gzf3 in cytoplasm |
| abstract | The invention discloses a method for positioning Gzf3 in cytoplasm to reduce urea accumulation of yellow wine yeast, belonging to the field of genetic engineering. The invention uses recombinant yellow wine yeast JNZ01(MATa, delta ura3, delta trp1, TOR1S1972R and delta fpr1) as an original strain, and performs fusion expression on GZF3 and FKBP12 on a genome and fusion expression on RPL13A and FRB on a plasmid, and the FKBP12 and FRB are combined by adding rapamycin. On the basis, Gzf3 is transferred to cytoplasm under the action of cytoplasmic localization protein Rpl13A to activate the transcription of related genes for urea utilization, so that the accumulation of urea in the fermentation process is reduced to 21.51% and reduced to 12.80 mg/L. |
| priorityDate | 2018-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.