| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2d6caadbc395b3d6ec4ecb6783f1a50d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24352 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
| filingDate |
2018-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99b86e9ed994616620b8645ed9117fec
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76c34aca2c7c930a94f1ad7bd08ea1b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11e0c25527d2db9d9084ff5cd8558f47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_030aa8a0f0a90b88ec4ba026cfd8d463
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f66e0120fdd8aaf33ffdbd9800d9ca7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_231561b580a49b5f501a70ec0b818669 |
| publicationDate |
2019-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-109517800-A |
| titleOfInvention |
A reconstituted ST cell that enhances the endogenous synthesis of cholesterol and its construction method and use |
| abstract |
The invention relates to the field of biotechnology, in particular to the field of transformation and construction of cell lines, and more particularly to a reconstructed ST cell that enhances the endogenous synthesis of cholesterol and a construction method and application thereof. This cell line was deposited in the General Microbiology Center of the China Microorganism Culture Collection Management Committee (CGMCC for short) on November 7, 2018, and the deposit number is CGMCC NO.16694. The reconstituted ST cell line that enhances the endogenous synthesis of cholesterol disclosed in the invention can grow with normal metabolism under the culture condition of serum-free medium. Experiments showed that there was no significant difference in the intracellular total cholesterol content of ST cells cultured normally under the condition of containing 10% serum, which laid a foundation for the proliferation of related viruses under the condition of serum-free culture. At the same time, taking the swine fever virus as an example, the virus proliferation titer of the reconstituted ST cells is much higher than that of the non-reconstituted original ST cells. |
| priorityDate |
2018-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |