http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109516969-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1088 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D311-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06 |
| filingDate | 2018-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109516969-B |
| titleOfInvention | Carboxyl-containing coumarin derivative and synthesis method and application thereof |
| abstract | The invention discloses a coumarin derivative containing carboxyl, a synthesis method and an application thereof, wherein the derivative is named as (Z) -2-cyano-3- (4- ((E) -3- (7- (4- (ethoxycarbonyl) piperazine-1-yl) -2-oxo-2H-chromene-3-yl) -3-oxoprop-1-en-1-yl) phenyl) acrylic acid in the Chinese, is named as (Z) -2-cyano-3- (4- ((E) -3- (7- (4- (ethoxycarbonyl) piperazin-1-yl) -2-oxo-2H-chromen-3-yl) -3-oxoprop-1-en-1-yl) phenyl) acrylic acid in the English, is named XI-2. The invention provides a method for specifically detecting cysteine by using derivative XI-2, which is characterized in that the content of cysteine is quantitatively detected by a fluorescence spectrophotometer in a PBS-DMSO (4:1, v/v, pH 7.4) solution. The detection process is circulated, simple, convenient, sensitive and quick, and the detection result is accurate. The invention also provides application of the derivative XI-2 in preparation of a cell cysteine detection reagent, namely the XI-2 efficiently permeates cell membranes under the regulation and control action of NEM to realize detection of cysteine in cytoplasm. |
| priorityDate | 2018-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.