| abstract |
The present invention relates to the field of biotechnology, in particular to the application of primer combinations in the species identification of sheep and/or the identification of sheep meat. The primers screened by the invention have high sensitivity and strong specificity, and the established detection method has the advantages of high accuracy, short detection time, and real-time observation of results, and can complete the distinction between sheep and goats only through the difference of short sequences. It only takes 1 hour from sample processing to reporting results, the operation is simple, and it has higher specificity than other PCR methods. At the same time, the present invention designed and screened the LAMP primers of sheep meat in the detection sample for the first time, and established the LAMP detection method of sheep meat, which filled the gap in this detection field at home and abroad. |