| abstract |
The invention belongs to the field of genetic engineering, and particularly relates to a salt stress inducible promoter, primers, expression vectors and applications thereof. The present invention includes promoter fragment cloning, constructing a promoter fragment vector, Arabidopsis genetic transformation, screening and detection of Arabidopsis resistant materials, GUS staining and RT-qPCR detection of GUS gene expression. The results show that the promoter fragment of the present invention can drive the expression of the GUS gene, and can be expressed in various parts such as roots, stems, leaves, flowers, etc., and the activity and driving efficiency of the promoter under abiotic stress induction conditions are also higher than those under normal conditions. The qPCR results also showed similar results, indicating that the promoter provided by the present invention can improve the salt tolerance of transgenic crops and has wide application value. |