| abstract |
The invention discloses a kind of sweet potato SPVD viruses to recombinate double CP fusion proteins, polyclonal antibody prepared therefrom, preparation method and its application, belongs to field of biotechnology.Sweet potato SPVD virus of the invention recombinates double CP fusion proteins, is that recombinant expression carrier is building up to by the fusion segment of sweet potato chlorotic stunt virus and Sweet Potato Feathery Mottle Virus CP gene, and conversion expression bacterial strain, inducing expression obtains.And the sweet potato SPVD virus of preparation is recombinated into double CP fusion protein immunization rabbits, the antiserum of acquisition is that sweet potato SPVD virus recombinates double CP polyclonal antibodies.The present invention recombinates double CP polyclonal antibodies by preparation sweet potato SPVD virus, can detect sweet potato chlorotic stunt virus and pinniform mottle virus simultaneously, improve detection efficiency, reduce testing cost. |