http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109490260-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2018-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109490260-B |
| titleOfInvention | Functional nucleic acid fluorescence sensor and application thereof in lead ion detection |
| abstract | The invention provides a low-background and temperature-stable functional nucleic acid fluorescence sensor, which comprises a molecular recognition element and a signal conversion element, wherein the molecular recognition element is a lead ion-dependent deoxyribozyme, the lead ion deoxyribozyme consists of a deoxyribozyme substrate chain and a deoxyribozyme chain, the nucleotide sequence of the deoxyribozyme substrate chain is shown as SEQ ID No.1 in a sequence table, and the nucleotide sequence of the deoxyribozyme chain is shown as SEQ ID No.2, SEQ ID No.3 or SEQ ID No.4 in the sequence table; the signal conversion element consists of Sub F of which the 5 'end is marked by a fluorescent staining group and Sub Q of which the 3' end is marked by a fluorescent quenching group, and the nucleotide sequences of the Sub F and the Sub Q are shown as SEQ ID No.5 and SEQ ID No.6 in the sequence table. The invention also provides an application of the fluorescent sensor in lead ion detection. The fluorescence sensor provided by the invention can improve the sensitivity of the existing biosensor method for detecting lead ions and the tolerance to the detection environment. |
| priorityDate | 2018-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.