http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109486815-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109486815-B |
| titleOfInvention | Artificial chimeric promoter and construction method thereof |
| abstract | The invention belongs to the technical field of genetic engineering, and discloses an artificial chimeric promoter and a construction method thereof; the pTobR and pWIN are combined by a fusion PCR method and an artificial combination element C1 is inserted into the combination to construct a chimeric promoter TC 1W; constructing a chimeric promoter TC1W into a pBI121 binary plant expression vector, and transferring TC1W into tobacco through agrobacterium mediation; GUS tissue staining and GUS protease activity determination are carried out on transgenic tobacco plants, and the expression mode of downstream GUS genes driven by the fusion promoter TC1W is explored. According to the invention, different promoter elements are combined to replace or redesign and construct a new artificial promoter, so that a new promoter with the characteristics of wide induction factors, low background activity, high expression intensity, quick expression starting and the like is constructed, and the expression of a downstream target gene can be better controlled. In the absence of inducing factors, the TC1W promoter exhibits root specificity only. |
| priorityDate | 2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.