http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109486800-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-2105 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate | 2018-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109486800-B |
titleOfInvention | Novel lysyl endopeptidase and preparation method thereof |
abstract | the invention discloses a novel lysyl endopeptidase, which is characterized in that a core peptide, a histidine tag and an arginine tail end of the novel lysyl endopeptidase are connected through appropriate flexible connecting peptides, so that the original enzyme activity is maintained, meanwhile, nanogram-level residual enzyme in a sample can be detected through an ELISA method, and a pure product of the novel lysyl endopeptidase can be obtained through purification through ion exchange chromatography. The invention also provides a preparation method of the lysyl endopeptidase, the recovery amount of a pure product of 107mg of the novel lysyl endopeptidase can be achieved after 1L of fermentation liquor is purified, and the method has industrial potential. |
priorityDate | 2018-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 225.