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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-22002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-63
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50
filingDate 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109486798-B
titleOfInvention A kind of separation method of papain crude enzyme
abstract The invention discloses a method for separating crude papain enzyme, comprising the following steps: (1) papain pretreatment; (2) wet bead milling and crushing; (3) freezing centrifugal preliminary filtration; 5) Saturated salting out. The technological process of the invention is more optimized, more papain milk can be obtained, browning can be effectively prevented in the preparation process, the sulfhydryl group of papain can be effectively protected, and the enzymatic activity is prevented from decreasing, and the enzymatic activity of the finally prepared papain can reach Above 600,000 u/g.
priorityDate 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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