http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109486782-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2018-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109486782-B |
| titleOfInvention | Method for improving sucrose phosphorylase expression efficiency through molecular chaperone co-expression |
| abstract | The invention discloses a method for improving the expression efficiency of sucrose phosphorylase by molecular chaperone co-expression, belonging to the technical field of biological engineering and enzyme engineering. According to the invention, the recombinant plasmid pET-20b-SPase and pGro7 are co-expressed, so that the molecular chaperone protein expressed by pGro7 can effectively reduce the formation of inclusion bodies, and the improvement of the soluble expression level and activity of the SPase is promoted. By optimizing the expression conditions and using a molecular chaperone co-expression system, the intracellular enzyme activity of the SPase reaches 24.33U/mL, and the specific enzyme activity reaches 6.58U/mg. |
| priorityDate | 2018-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 330.