http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109486737-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-01071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y103-01012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-227 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-22 |
filingDate | 2018-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109486737-B |
titleOfInvention | Recombinant escherichia coli with high L-tryptophan yield and construction method thereof |
abstract | The invention discloses a recombinant escherichia coli with high L-tryptophan yield and a construction method thereof, belonging to the technical field of genetic engineering. The invention uses Escherichia coli CICC10303 as an original strain, and adopts CRISPR-Cas9 gene editing technology to replace shikimic acid kinase coding gene aroK promoter as a strong promoter T7; replacing the promoter of the encoding gene pheA of the prephenate dehydrogenase with a weak promoter tac; knocking out the tryptophan transporter coding gene mtr and preventing the tryptophan from being transported back to the cell in the fermentation process. Finally, the Escherichia coli genetic engineering bacteria accumulating the L-tryptophan are obtained, the yield reaches 36g/L, and a foundation is laid for further metabolic engineering transformation of Escherichia coli to produce the L-tryptophan. |
priorityDate | 2018-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 527.