http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109479725-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2019-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109479725-B |
| titleOfInvention | Method for rooting populus diversifolia tissue culture seedlings outside bottles |
| abstract | The invention relates to a method for rooting populus diversifolia tissue culture seedlings outside bottles. The method comprises the following steps: removing the culture bottle of the populus diversifolia tissue culture seedlings with the seedling height of 6-8 cm from the culture room, closing the bottle and hardening the seedlings for 7-10 days; opening the bottle and hardening off the seedlings for 5 to 7 days; after the tissue culture seedlings of the populus diversifolia are subjected to induced rooting pretreatment by a liquid culture medium, transplanting the seedlings into a seedling culture tray containing a rooting medium, completely watering root fixing water after the seedlings are planted, and covering the seedling culture tray with a plastic film or a transparent container for moisturizing; spraying 2 times with an improved 1/2MS liquid culture medium every day in the early stage, wherein the temperature of a culture room is 25-30 ℃, the illumination intensity is 2000-2500 lux, and after indoor culture is carried out for 20-30 days, the rooting length of the populus diversifolia tissue culture seedlings reaches 5-7 cm; and finally, transplanting in a field. The invention has the advantages that: the tissue culture seedling ex-bottle rooting technology reduces the links of preparation, sterilization, inoculation and the like of a rooting culture medium, simplifies the production procedure, shortens the seedling culture time, and can shorten the overall seedling culture time by more than 20 days; the final rooting rate is 99.3%, and the survival rate is 98%. |
| priorityDate | 2019-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.