| abstract |
The present invention provides methods, compositions, mixtures and kits using deoxynucleoside triphosphates comprising a 3'-nucleoside capped with a group comprising methylene disulfide as a cleavable protecting group A detectable label at the O position and a nucleobase reversibly attached to the deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to sequencing by synthesis. |