http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109468313-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2019-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109468313-B |
| titleOfInvention | Light-controlled functionalized capillary tube for nucleic acid extraction and preparation method and application thereof |
| abstract | The invention discloses a light-regulated functionalized capillary tube for nucleic acid extraction and a preparation method and application thereof, belonging to the technical field of biological detection. According to the invention, 3-aminopropyltriethoxysilane, 4-bromomethyl-3-nitrobenzoic acid and 4-amino-1-butanol are sequentially modified on the inner surface of the capillary by a chemical modification method, so that the inner surface of the capillary is provided with a large amount of positive charges, and nucleic acid with electronegativity can be adsorbed; and under the irradiation of 302nm ultraviolet light, the chemical bond generated by the reaction of the 4-bromomethyl-3-nitrobenzoic acid and the 4-amino-1-butanol is broken, so that the nucleic acid adsorbed on the inner surface of the capillary is released into the liquid phase again for the next detection. The invention greatly simplifies the operation process of nucleic acid separation and extraction, shortens the time of nucleic acid separation and extraction, and can utilize ultraviolet irradiation to re-release the extracted nucleic acid molecules into the liquid phase for subsequent storage or detection. |
| priorityDate | 2019-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.