http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109468256-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2035-115 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-741 |
| filingDate | 2018-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109468256-B |
| titleOfInvention | Probiotic clone strain integrating four-copy F18 pilus operon gene and double-copy F4 pilus operon gene and construction method |
| abstract | The invention relates to the technical field of biology, in particular to a probiotic clone strain integrating a four-copy F18 pilus operon gene and a two-copy F4 pilus operon gene and a construction method thereof, which comprises the following steps: pTargetT- X ::P tet F18 and pTargetT- X ::P tet Constructing a F4 recombinant plasmid; by the construction of a probiotic clone strain integrating a four-copy functional F18 pilus operon gene and a double-copy functional F4 pilus operon gene, a nonresistant four-copy F18 pilus gene and double-copy F4 pilus gene compound integrated clone strain is obtained. The invention has the beneficial effects that: the recombinant strain can effectively improve the adhesion performance to porcine intestinal passage epithelial cells; the orally immunized mice were able to produce immune sera, antibodies to IgG against the F18 fimbriae and against the F4 fimbriae; the serum of the mice can obviously reduce F18 after oral immunization + And F4 + Adherence of the strain to the porcine intestinal continuous cell line IPEC-J2. |
| priorityDate | 2018-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.