http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109444434-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2446-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-47 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54346 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2018-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109444434-B |
titleOfInvention | Method for detecting antibody by double-antigen sandwich |
abstract | The invention relates to a method for detecting antibodies by a double-antigen sandwich, which forms a solid phase support-a first antigen Ag 1 Antibody to be detected-second antigen Ag 2 The form of (1) wherein Ag completes the assay 2 Coupled with a label for displaying signal intensity, the method comprising the steps of: 1) mixing Ag with water 1 、Ag 2 Contacting with a sample under conditions sufficient for antibody/antigen binding reactions to occur to form an immune complex; wherein in terms of mole number, Ag 1 Is more than Ag 2 (ii) a 2) Washing away unbound antibody to be detected; 3) adding Ag 2 And allowing it to bind to the remaining antigen binding sites in the immune complex; 4) detecting the marker to indicate the presence and/or amount of the antibody to be detected. Compared with the prior art, the method provided by the invention not only ensures the sensitivity of low-value detection, but also solves the hook effect and reduces the rate of missed detection. |
priorityDate | 2018-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 466.