| abstract |
The invention discloses a method for detecting curcumin compounds in Cinnamon vulgaris, comprising the following steps: (1) preparation of a reference solution; (2) preparation of a test solution; (3) determination: the chromatographic conditions of high performance liquid phase are: AgilentSB-C 18 chromatographic column, mobile phase is acetonitrile-0.25% glacial acetic acid, detection wavelength is 415-425nm, column temperature is 25-35℃, flow rate is 0.8-1.2mL/min, injection volume is 10μL. The reference substance of curcuminoids was selected from curcumin, demethoxycurcumin and bisdemethoxycurcumin. The invention has good separation effect, high detection sensitivity and good reproducibility; the curcumin content in Guiyujin can be characterized by high performance liquid chromatography, which can provide a basis for industrial extraction and detection, and is very beneficial to the quality evaluation and quality control standards of Guiyujin Establish. |