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filingDate 2018-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7b7af72824cf0c17f549ccb1110e5ea
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publicationDate 2019-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109439762-A
titleOfInvention Molecular markers and methods for the identification of Pseudomonas japonicus and S.
abstract The invention provides a molecular marker and a method for identifying the jellyfish and the three-piece fish, and relates to the field of group identification of the three-piece fish genus. The first identification molecular marker primer pair of the present invention is A8. The second identification molecular marker primer pair of the present invention is A11. The methods of identification of Zhuxing tilapia and three pieces of fish: 1. Extraction of sample DNA; 2. PCR detection; 3. Agarose gel electrophoresis of PCR amplification products; 4. Judgement of PCR results. The method of the invention simplifies the steps of identifying the germplasm molecules of the pearl star three-piece fish and the three piece fish, and distinguishes the pearl star three piece fish from the three piece fish according to the visualization of the results of agarose gel electrophoresis, and has the advantages of simple steps and stronger operability. , The equipment requirements are lower, the identification process is faster, and the identification results are more accurate. The method of the invention can avoid the mixing of germplasm caused by artificial propagation and protect germplasm resources.
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