http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109439680-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-78 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate | 2018-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109439680-B |
titleOfInvention | Method for preparing antibacterial recombinant protein |
abstract | The invention belongs to the technical field of biology, and relates to a method for designing, synthesizing, expressing, separating and purifying an antibacterial protein gene, in particular to a method for preparing an antibacterial recombinant protein based on type III human fibronectin (Fn3) and sea snake antibacterial peptide (Hc-CATH). The method comprises the steps of recombining a nucleotide sequence of a coded III type human fibronectin (Fn3) with a nucleotide sequence of a coded sea snake antibacterial peptide (Hc-CATH) through coding 10-15 asparagine (Asn) sequences by gene recombination to synthesize a III type human fibronectin Fn3 and sea snake antibacterial peptide Hc-CATH fusion gene, and efficiently expressing by utilizing an escherichia coli expression system to prepare the fusion protein with antibacterial activity. The invention has the beneficial effect that the fusion protein with antibacterial activity can be simply, quickly and efficiently prepared. |
priorityDate | 2018-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.