| abstract |
The invention discloses a preparation method of a filler for treating osteoarthritis by using mixed stem cells, which comprises collecting and preparing adipose tissue, collecting cord blood, collecting cell tissue, separating and culturing of adipose stem cells, separating and culturing umbilical cord mesenchymal stem cells, and cells. The chondrocytes were mixed and induced to form, diluted with physiological saline, and platelet-rich plasma was prepared and added, and a filler was prepared. The beneficial effects of the present invention are: the temperature of the incubator used for the culture of the adipose stem cells and the umbilical cord mesenchymal stem cells is set to 37 ° C, and the inside is filled with a CO 2 gas content of 5%, which satisfies the components required for stem cell growth and reproduction, in the mixed cells. Magnetic nanospheres were added to the solution and ultrasonically assisted cell dispersion at 37 ° C to further enhance the process of differentiation, increase the number and quality of induced differentiation of chondrocytes, and mix the cells with adipose stem cells and umbilical cord mesenchymal stem cells. The content ratio is 2:1, which is more conducive to the induction of chondrocytes. |