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publicationDate 2019-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109416354-A
titleOfInvention Osmotic Imbalance Method for Bilayer Formation
abstract A method of forming multiple lipid bilayers on an array of assay cells in a nanopore-based flow chip is disclosed. Each assay cell includes wells. A first salt buffer solution having a first osmolality is flowed through an assay cell in the nanopore-based flow chip to substantially fill the wells in the assay cell with the first salt buffer solution. A lipid and solvent mixture is flowed through the assay cell to deposit a lipid film on the well, the lipid film enclosing the first salt buffer solution in the well. flowing a second salt buffer solution having a second osmolality over the pores to reduce the thickness of the lipid membrane, wherein the second osmolality is lower than the first osmolality osmolarity, thereby creating an osmotic imbalance between the first volume inside the pore and the second volume outside the pore.
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