http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109415419-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-31 |
| filingDate | 2017-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aefaa2e12b28f1769b738e619c5248b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc4fb28e23c6d80610df3d556581305d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_152c511e3a21bc88983538f60abc2888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26263739ef59ba93de655122c59d7c15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_572ef4d69954e446ec78b513922d0e24 |
| publicationDate | 2019-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109415419-A |
| titleOfInvention | Long-lived alpha-hemolysin nanopores |
| abstract | Described herein are variants of alpha-hemolysin having at least one amino acid substitution at H35G, E111N, M113A and/or K147N in the mature, wild-type alpha-hemolysin amino acid sequence. In certain examples, the variant may have a substitution at E111S, M113S, T145S, K147S, or L135I in the mature alpha-hemolysin amino acid sequence. Alpha-hemolysin variants may also include substitutions at H144A of mature, wild-type alpha-hemolysin and/or a series of glycine residues spanning residues 127-131. Also provided are nanopore assemblies comprising alpha-hemolysin variants, the assemblies having increased nanopore lifetime. Further, variants are provided that, in addition to providing increased lifetime, also provide reduced penetration time. Thus, the variants provided herein increase nanopore lifetime and improve the efficiency and accuracy of DNA sequencing reactions using nanopores comprising the variants. |
| priorityDate | 2016-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 99.