| abstract |
The invention relates to a method for observing the three-dimensional distribution of cells in micro-tissues and counting the number of cells in the technical field of microscopic observation of biological tissues. Transparency treatment; then move the tissue material together with the clear liquid to a special dish for confocal microscope, scan and take pictures layer by layer with a laser confocal microscope, obtain images of cell distribution in each layer of the whole tissue, observe the three-dimensional distribution of cells, and then pass The Imaris software processes the image data of the cell distribution in each layer, reconstructs the three-dimensional model of the cell tissue, observes the three-dimensional distribution of the immunofluorescently labeled cells in the three-dimensional three-dimensional model, and then uses the Imaris Bitplane Spot detection and analysis to calculate the exact number of immunofluorescently labeled cells. The method of the present invention maintains the integrity of the tissue to be observed, and the clarity and accuracy of the observed cell distribution are high, and the statistical result of the number of cells is more accurate. |