http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109406704-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 |
| filingDate | 2018-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109406704-B |
| titleOfInvention | Method for measuring contents of various organic acids in tobacco and tobacco products |
| abstract | The invention belongs to the technical field of tobacco analysis, and particularly relates to a method for measuring contents of various organic acids in tobacco and tobacco products. The measuring method of the present invention comprises the steps of: uniformly mixing a sample to be detected, a saponification reagent and an internal standard substance, refluxing at the temperature of 60-70 ℃, then carrying out solid-liquid separation, and then carrying out reduced pressure concentration on a filtrate until the filtrate is dried and then dissolving in water to obtain a solution A; adjusting the pH value of the solution A to 1.5-2.5 at the temperature of 0 ℃, and then adding an extracting agent for extraction; adding a silanization derivatization agent into the extract liquid for derivatization reaction, and then detecting and analyzing by using gas chromatography-tandem mass spectrometry. The invention establishes a saponification-silanization-GC-MS/MS analysis method for the first time, can be used for analyzing 53 organic acids in a sample, and has more varieties and more comprehensiveness of target analytes including low molecular weight organic acids, semi-volatile organic acids and higher fatty acids; the GC-MS/MS analysis parameter adopted in the invention has higher sensitivity, and can realize the detection of organic acid with lower content in tobacco and tobacco products. |
| priorityDate | 2018-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 156.