| abstract |
The invention belongs to the technical field of radiation protection, and relates to a method for detecting the influence of electron beam irradiation on the expression of IGF-related proteins in mice. The detection method includes mouse IGF-related protein expression analysis and/or mouse IGF-related protein expression distribution analysis and/or cell apoptosis analysis in mouse liver tissue, and mouse IGF-related protein expression analysis includes the following steps: : (1) total protein extraction and concentration determination of mouse liver; (2) total protein electrophoresis and Western-Blot detection of mouse liver; (3) molecular weight and expression analysis, expression distribution analysis of mouse IGF-related proteins, mouse liver The analysis of cell apoptosis in the tissue includes the following steps: (A) preparation; (B) confocal microscope observation. Using the detection method of the present invention, the mechanism of radiation-induced damage and cancer can be explored, and the results can provide a theoretical basis for the early damage of the liver by radiation, and provide a new target index for radiotherapy. |