http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109402239-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109402239-B |
| titleOfInvention | Hands-free direct amplification reagent for real-time fluorescent quantitative PCR and application thereof |
| abstract | The invention provides a hand-free direct amplification reagent for real-time fluorescent quantitative PCR and application thereof, wherein the direct amplification reagent comprises the following components: sodium hydroxide (NaOH), Cyvantin (Surfactin), dimethyl sulfoxide (DMSO), sucrose, chelex-100. When the kit is used, nucleic acid extraction and purification are not required to be carried out on a sample, and the sample and the reagent are mixed according to the ratio of 1: 1, and the mixed solution can be directly used for fluorescence PCR detection. The invention is suitable for extracting nucleic acid of human genome, bacteria and virus in samples such as oral swab, nasopharyngeal swab, serum, plasma, genital secretion and the like; the invention is not only suitable for probe method fluorescence PCR, but also suitable for dye method PCR; the kit is suitable for various clinical purposes, can simply and quickly complete detection, and has the advantages of low cost, simple and convenient operation, accurate result and the like. |
| priorityDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 21.