http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109402182-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-42 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-42 |
| filingDate | 2018-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109402182-B |
| titleOfInvention | Method for preparing 10-hydroxy-2-decenoic acid by using resting cells of escherichia coli engineering bacteria |
| abstract | The invention relates to a method for preparing 10-hydroxy-2-decenoic acid by using resting cells of escherichia coli engineering bacteria, which comprises the following steps: (1) constructing an escherichia coli engineering bacterium containing a recombinant plasmid pET-28 a-ydiI; (2) preparing an induced cell from an escherichia coli engineering bacterium containing a recombinant plasmid pET-28 a-ydiI; (3) culturing the induced cells in a transformation culture medium to obtain resting cells, and then adding 10-hydroxydecanoic acid into the culture medium to prepare 10-hydroxy-2-decenoic acid; the invention discloses positive recombinant escherichia coli containing thioesterase, which is modified by genetic engineering for the first time, and can produce 10-hydroxy-2-decenoic acid through resting cell fermentation after special induction treatment, thereby realizing the mass biosynthesis of 10-hydroxy-2-decenoic acid. |
| priorityDate | 2018-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 138.