http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109402156-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b2f4249e501141bfa522f73bfe661ef |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-00022 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d024e2aa5e747118573a9320611e1c18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc1b0dc8f4846c7682c61b3772be767b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_219fe0e2c92d18f2af054c1ad4ecafb1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4ca6b02201227db7608422675a6e75a |
publicationDate | 2019-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109402156-A |
titleOfInvention | A temperature-controlled lysis plasmid and its construction and application in the preparation of bacterial sloughs |
abstract | The invention relates to a temperature-controlled lysis plasmid and its construction and application in the preparation of bacterial sloughs. The nucleotide sequence of Kn is SEQ ID NO: 1. Compared with the prior art, the present invention has the following advantages: the constructed temperature-controlled lysis plasmid has kanamycin resistance and can be used for kanamycin-sensitive The bacterial slough construction of the pathogenic bacteria, at the same time, compared with the suicide plasmid with kanamycin resistance, the plasmid constructed in the present invention only needs one round of resistance screening to obtain a positive strain for the slough preparation, The operation is simple and convenient, which is favorable for popularization, and the plasmid provided by the invention has a small genome (3887bp), is easy to stably replicate and inherit in the host bacteria, and has higher reliability. |
priorityDate | 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.