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filingDate 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d024e2aa5e747118573a9320611e1c18
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publicationDate 2019-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109402156-A
titleOfInvention A temperature-controlled lysis plasmid and its construction and application in the preparation of bacterial sloughs
abstract The invention relates to a temperature-controlled lysis plasmid and its construction and application in the preparation of bacterial sloughs. The nucleotide sequence of Kn is SEQ ID NO: 1. Compared with the prior art, the present invention has the following advantages: the constructed temperature-controlled lysis plasmid has kanamycin resistance and can be used for kanamycin-sensitive The bacterial slough construction of the pathogenic bacteria, at the same time, compared with the suicide plasmid with kanamycin resistance, the plasmid constructed in the present invention only needs one round of resistance screening to obtain a positive strain for the slough preparation, The operation is simple and convenient, which is favorable for popularization, and the plasmid provided by the invention has a small genome (3887bp), is easy to stably replicate and inherit in the host bacteria, and has higher reliability.
priorityDate 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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