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filingDate 2018-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109402049-B
titleOfInvention A preparation method of tissue engineered adipose stem cell sheet with cartilage differentiation potential that can be used for cartilage damage repair
abstract The invention discloses a method for preparing tissue engineering adipose stem cell sheets with cartilage differentiation potential that can be used for cartilage damage repair. The invention prepares a specific culture system, and the ASCs sheets obtained by the cell sheet culture technology are extracellular Intact sheet-like structure of the matrix with chondrogenic differentiation potential. Since only tissues composed of pure cells are transplanted into the body, the use of carriers such as scaffolds and the adverse reactions caused by them are avoided. The ASCs sheet retains the extracellular matrix and contains important cell surface proteins such as ion channels, growth factor receptors, and connexins, which can better maintain the coordination of functions between cells, which has the incomparable advantages of traditional tissue engineering techniques. The complete lamellar structure is conducive to the retention of cells at the transplant site, which greatly improves the utilization rate of cells and the activity of transferred cells.
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