http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109400673-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-54 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K5-0215 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-037 |
| filingDate | 2018-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109400673-B |
| titleOfInvention | Method for extracting and purifying glutathione by metal ion precipitation-cyclodextrin inclusion |
| abstract | The invention discloses a method for extracting and purifying glutathione by metal ion precipitation-cyclodextrin inclusion. The method comprises the following steps: 1) pulverizing embryo of sea rice, lyophilizing at low temperature to water content of 5-8%, and removing lipid substances from embryo of sea rice by supercritical carbon dioxide fluid extraction; 2) extracting the degreased rice embryo with hot water, and concentrating the supernatant to obtain a glutathione crude product; 3) dissolving the glutathione crude product in hot water, adding acetic acid to adjust the pH value of the solution to 4-5, slowly adding zinc acetate, stirring at 100-150rpm for reaction to generate a glutathione zinc salt precipitate; maintaining the pH value of 4-5, keeping the temperature at 30 ℃, reacting for 4h, and fully reacting and aging; 4) centrifuging at high speed, removing supernatant, adding the precipitate into 0.5% EDTA solution, and stirring at room temperature to dissolve; 5) slowly adding 10% beta-cyclodextrin water solution, heating to 45 deg.C, stirring to generate precipitate, centrifuging to remove precipitate, and concentrating the supernatant under reduced pressure to obtain purified glutathione. The supercritical extraction degreasing does not use common organic solvents such as petroleum ether normal hexane and the like, avoids organic residue pollution and prevents the interference of the marine rice germ lipid substances on the separation result. |
| priorityDate | 2018-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.