| abstract |
The present invention provides a serum urea determination reagent and application thereof. The reagent includes a reagent R1 and a reagent R2; wherein, the raw materials of the reagent R1 include Capso, NADH and EDTA.Na 2 , and the raw materials of the reagent R2 include HEPES , ɑ-ketoglutarate, urease, glutamate dehydrogenase, EDTA.Na 2 , reducing agent and BSA; the present invention provides an effective buffer capacity by optimizing the reagent formula and adjusting the pH of the buffer, improving the NADH Stability, reducing the oxidation of tool enzymes, synergistic synergy of each component, solving the problem of poor stability of blank absorbance change rate, providing the reliability of clinical test results in hospitals, with broad prospects and huge market value. |