http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109385469-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd4309bd382ea4995ab5a085a4b8e92c |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
filingDate | 2018-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f06d5f6f31174dbba61b7fdea46e2eab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cde6536d8b7e53bed8fb5cf7ed445054 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bcaa03e76062ddd428a840cba77c85f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4729adf62be34d325f6f2f404cc495c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f08edb108790954380c2530b6643c8ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94bf93cba8c22310694fb6fc322bad0e |
publicationDate | 2019-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109385469-A |
titleOfInvention | A high-sensitivity double-stranded circulating tumor DNA detection method and kit |
abstract | The invention relates to the technical fields of biotechnology and DNA detection, in particular to a high-sensitivity double-stranded circulating tumor DNA detection method and kit. The method of the invention includes DNA end repair and end tailing reaction on plasma free DNA, ligation reaction with UMI-containing adaptor, AMPure XP magnetic bead purification, qPCR reaction, library high-fidelity PCR amplification using qPCR results, and library Carry out steps such as oncogene targeted capture. The method of the invention can obtain higher library yield, overcome the defect of unstable library quality caused by ctDNA quality difference, fundamentally improve the detection sensitivity and accuracy, and reduce the detection false positive rate. Therefore, the method of the present invention can be used to prepare various tumor tracer or tumor screening kits to meet various clinical and research needs in the biomedical field. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021027810-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111088328-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112410406-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111549131-A |
priorityDate | 2018-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.